Corrigendum Over-expression of recombinant proteins with N-terminal His-tag via subcellular uneven distribution in Escherichia coli

The authors apologise for this error. Figure 4. His-tag effect on uneven distribution and activity of chloramphenicol acetyltransferase (CAT), neopullulanase (BCN) and esterase 1767 (A) Distinct uneven distribuiton of proteins with N-terminal His-tag were observed by immunofluorescence staining using anti-His-tag antibody. As a negative conrol, unlocalized patterns of mBFP (without His-tag) using anti-mBFP antibody was shown in this figure. All constructs were expressed under the same conditions using the expression vector pTrc99A. (B) Comparison of distrubiton pattern of proteins with Nand C-terminal His-tag, or without tag under the same culture conditions. All immunofluorescent images were observed by using anti-mBFP antibody. Werstern blot was also conducted using the same antibody as a control for protin expression under the same condition. (C) Zymogram assay for esterase 1767 (left pannel) and maximum inhibitory concentration (MIC) assay for CAT (right pannel). For zymogram assay, an aliguot (10 μg) of the crude enzymes was analyzed on a 11% native-PAGE and then stained with α-naphthyl acetate and Fast Blue RR. Lane 1, empty vector; lane 2, 1767; lane 3; His-1767; lane 4, 1767-His. MIC assay showed differences in inhibitory zone against 6 mm paper impregnated with the same concentration of chloramphenicol (10 ppm). Acta Biochim Biophys Sin, 2015, 47(8), 661 doi: 10.1093/abbs/gmv069 Corrigendum